Part:BBa_K4613301

pET-29a(+)-C3

This composite part was constructed to analyze the function of C3 and the intensity of the T7 lac promoter. The composite part can be directly imported into plasmid and express induced C3 with IPTG.

Fig. 1 (a)The plasmid map of pET29a(+)-C3. (b)SDS-PAGE analysis of the purified protein C3 in E. coli BL21 (DE3) cultured in LB medium express protein for 3 hours at 37℃. Lane M: protein marker. Lanes 1-7: flow through and elution containing 20, 50, 50, 100, 100, 250, 250 mm imidazole, respectively.

Reference

1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1645
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1620
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]