Part:BBa_K4960002

pvc2

Properties

Pvc2 is a part of the sheath.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1,Figure2)

Figure1:PVC illustrations
Figure2:Payload illustrations

The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. The PVC sheath body is predominantly made of Pvc2 hexamers,(Figure.3) and deletion of the main sheath protein Pvc2 results in sheath-free particles.
Among the three, Pvc2 has the simplest structure, which closely relates to the R-type pyocin sheath protein. Pvc2 is consisted of two major domains (domain I and II)
Pvc2 starts to assemble along the inner tube for the maturation of PVC particles with Pvc3 at the end of assembly.
(Figure.4)

Figure3.The proteins consisting of the pvc
Figure4.The protein pvc2 encodes Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr