Coding

Part:BBa_K4960008

Designed by: Ruoxi Lv   Group: iGEM23_NUDT-CHINA   (2023-10-10)
Revision as of 18:56, 10 October 2023 by Aaachengcheng (Talk | contribs)


pvc8

Properties

PVC8 is a part of PVC Spike.

Usage and Biology

A part of the simplified architecture of T4 phage baseplate lying in the PVC syringe with PVC5, PVC7 and PVC10. One part involved in the association between the wedges and the central hub. A fusion protein of T4 gp5 and gp27 homologs. Two sides of hexameric rings are found opposite surface charges, PVC8 included Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1213
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 511
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023.
[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019;

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