Part:BBa_K4613168

PAL

Based on its architecture comprising Per-ARNT-Sim (PAS), AmiR and NasR transcription antitermination regulators (ANTAR) and LOV domains, the protein indentified in the gram-positive actinobacterium Nakamurella multipartita is dubbed PAL.The light-activated, sequence-specific binding of PAL to its RNA aptamer (motif 58.16, BBa_K4613453) can be principally harnessed to control bacterial expression at the mRNA level.

Fig.1 The structure of PAL its dark-adapted state and the functional analysis of PAL.

Reference

1. Weber A M, Kaiser J, Ziegler T, et al. A blue light receptor that mediates RNA binding and translational regulation[J]. Nature Chemical Biology, 2019, 15(11): 1085–1092.
2. Ranzani A T, Wehrmann M, Kaiser J, et al. Light-Dependent Control of Bacterial Expression at the mRNA Level[J]. ACS Synthetic Biology, 2022, 11(10): 3482–3492.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 235
  Illegal PstI site found at 904
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 235
  Illegal PstI site found at 904
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 235
  Illegal PstI site found at 904
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 235
  Illegal PstI site found at 904
  Illegal AgeI site found at 280
  Illegal AgeI site found at 565
  Illegal AgeI site found at 775
  Illegal AgeI site found at 886
  
• 1000
  COMPATIBLE WITH RFC[1000]