Coding

Part:BBa_K4979002

Designed by: Xiong Jiameng   Group: iGEM23_Thinker-Shenzhen   (2023-10-09)
Revision as of 13:47, 9 October 2023 by Registry (Talk | contribs)

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alginate lyase AL2 +cellulase Bgls

We decide to express the alginate lyase AL2 to degrade alginate, which is a complex polysaccharide composed of sulfated alginate and mannuronic acid. Alginate lyase is capable of cleaving the glycosidic bonds between the sulfated alginate and mannuronic acid, thereby facilitating the degradation of alginate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 940
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 74


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Categories
Parameters
None