Coding

Part:BBa_K4613015

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 06:34, 9 October 2023 by Registry (Talk | contribs)

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T3-YFP

T3 (BBa_K4613011) and C3 (BBa_K4613012) can form protein complexes by elastin-like polypeptides(ELPs) monomers containing SpyTags and SpyCatchers. Different functional proteins can be incorporated into the polymeric scaffolds in a flexible manner due to its programmability. In this part, NAU-CHINA 2023 incorporated Yellow Fluorescent Protein(YFP). We fused YFP into T3 to immobilize the fluorescent protein and test the combination between T3 and C3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1589
    Illegal SapI site found at 8


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