Part:BBa_K4613014
T3-ADH3
T3(BBa_K4613011) and C3(BBa_K4613012) could form protein complexes by elastin-like polypeptides(ELPs) monomers containing SpyTags and SpyCatchers. If you want to learn about the detailed introduction of T3 and C3, you can click the link below. https://parts.igem.org/Part:BBa_K4164011 https://parts.igem.org/Part:BBa_K4164012 Different functional proteins can be incorporated into the polymeric scaffolds in a flexible manner due to its programmability. In this part, NAU-CHINA 2023 incorporated ADH3, which is the most efficient OTA-detoxifying enzyme reported thus far and can hydrolyze OTA to nontoxic ochratoxin α(OTα) and L-β-phenylalanine(Phe). Moreover, its soluble protein expression of ADH3 in Escherichia coli has been realized. We fused ADH3 into T3 to immobilize the enzyme and increase the stability and sustainable production of ADH3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 940
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1675
Illegal AgeI site found at 1363
Illegal AgeI site found at 1525 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 8
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