Device

Part:BBa_K4593018

Designed by: Jianfei Song   Group: iGEM23_BNDS-China   (2023-10-08)
Revision as of 21:04, 8 October 2023 by Jianfei Song (Talk | contribs)


Endolysin combinative expression circuit in B. subtilis

This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vitro. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract.

Detailed characterization of each endolysin is documented in their respective basic parts.

Please see the design page for usage instructions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2228
    Illegal NgoMIV site found at 3060
    Illegal NgoMIV site found at 3734
    Illegal AgeI site found at 2097
    Illegal AgeI site found at 3204
    Illegal AgeI site found at 3432
    Illegal AgeI site found at 3603
    Illegal AgeI site found at 3716
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None