Coding

Part:BBa_K200028

Designed by: Charles Fracchia, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)
Revision as of 23:04, 18 October 2009 by CharlesFracchia (Talk | contribs)

Protease resistant PAH

The Homo sapiens endogenous enzyme is synthesised by the liver. However, as part of the Imperial College iGEM 2009 project, [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator], the enzyme is released in the intestine of the individual. The presence of proteases in the intestine was therefore a serious problem which would jeopardise the viability of the curative enzyme. We therefore performed site directed mutagenesis in order to alter a serine residue (Ser16) to a glutamine as this change has previously been correlated with resistance against intestinal proteases . This will allow safe passage through the stomach and gut in order to carry out function as required by Imperial College's 2009 iGEM project, [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


<biblio>

</biblio>

[edit]
Categories
Parameters
None