Part:BBa_K4632002

Cry3A-like toxin

Bacillus thuringiensis UTD-001, as well as the protoxin and toxin of this isolate, could be used to control pests such as fire ants, carpenter ants, Argentine ants, and Pharaoh's ants, including Solenopsis invicta(S. Invicta). Cry3A-like toxin isolated fromUTD-001 have been shown to be toxic to S.Invicta.(Bulla and Candas, 2003)

A. How does it work?

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It has been shown that after treatment with papain in vitro, the Cry3A-like toxin prototoxin (72.9 KD) forms an active toxin (66.6 KD) that is toxic to S. Invicta.(Bulla and Canda, 2003)

B. Eco-friendly and Safe

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Bt (Bacillus thuringiensis) is widely recognized as a safe and environmentally benign insecticide. And the Bt toxin Cry3A-like protein we used is Eco-friendly and Safe.(see more detail on [1];https://2023.igem.wiki/scau-china/safety&#65289

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1813
  Illegal PstI site found at 295
  Illegal PstI site found at 304
  Illegal PstI site found at 1456
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1813
  Illegal PstI site found at 295
  Illegal PstI site found at 304
  Illegal PstI site found at 1456
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1813
  Illegal BglII site found at 533
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1813
  Illegal PstI site found at 295
  Illegal PstI site found at 304
  Illegal PstI site found at 1456
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1813
  Illegal PstI site found at 295
  Illegal PstI site found at 304
  Illegal PstI site found at 1456
  Illegal AgeI site found at 1495
  
• 1000
  COMPATIBLE WITH RFC[1000]