Composite

Part:BBa_K4634006:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-03)
Revision as of 05:19, 7 October 2023 by NgSweeHong (Talk | contribs) (→‎References)

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Histag OmpA mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

Constructed on PET-28a-Histag-GST-mCherry (lab stock) through PCR synthesis.

References

[1] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.