Plasmid

Part:BBa_K4634007:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-03)
Revision as of 05:15, 4 October 2023 by NgSweeHong (Talk | contribs) (→‎References)

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pTargetF with gRNA designed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 198
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 342
    Illegal BamHI site found at 186
    Illegal XhoI site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1137
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 122


Design Notes

NA


Source

Lab stock

References

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. 10.1128/AEM.04023-14 PubMed 25636838