Tag

Part:BBa_K4634005

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-09-25)
Revision as of 04:52, 4 October 2023 by NgSweeHong (Talk | contribs)

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6xHis affinity tag

The lac operator used in pET28a is a specific DNA sequence derived from the lac operon of Escherichia coli. It serves as a regulatory element in the plasmid's gene expression system. The lac operator is the binding site for the Lac repressor protein (LacI). When LacI binds to the operator, it blocks the transcription of genes downstream of the operator, including the T7 promoter that drives the expression of the target gene in pET28a.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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