Part:BBa_K4839010
(EAAAK)3 linker
(EAAAK)3 linker is a kind of rigid linker used in our study. In our experiment, we use this linker to connect IRF4 and GFP. At the same time, we also wish these two proteins have no other interaction between each other.
Figure1. The function of anti-IRF4 BioPROTAC
This figure shows that the IRF4 and GFP is connected by the (EAAAK)3 linker. This linker is a rigid linker, as a result, we wish this linker will prevent the intereaction between IRF4 and GFP. At the same time ,the linker can keep a realtive long distance between the two protein, which will prevent the nagative interaction occurs during the critical ubiquitination process. We delibrately extend the linker to lower the possibility of the unexpected interaction.
[1] Lim S , Khoo R , Peh K M ,et al.bioPROTACs as versatile modulators of intracellular therapeutic targets including proliferating cell nuclear antigen (PCNA)[J].Proceedings of the National Academy of Sciences, 2020(11).DOI:10.1073/PNAS.1920251117.
[2] Békés, M., Langley, D.R. & Crews, C.M. PROTAC targeted protein degraders: the past is prologue. Nat Rev Drug Discov 21, 181–200 (2022). https://doi.org/10.1038/s41573-021-00371-6
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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