Part:BBa_K4839006
vhhGFP4 (GFP Antibody frgement)
We firstly make use of the vhhGFP4, an efficient anti-GFP antibody peptide as our preliminary validation. vhhGFP4 can specifically target to the GFP-IRF4 protein, and then the SPOP component will recruit the ubiquitin and initiate the ubiquitination of GFP-IRF4, which will subsequently led to the degradation of this target protein (Figure 1). We prove this process through our experiment.
Figure1. The function of anti-GFP BioPROTAC
[1] Lim S , Khoo R , Peh K M ,et al.bioPROTACs as versatile modulators of intracellular therapeutic targets including proliferating cell nuclear antigen (PCNA)[J].Proceedings of the National Academy of Sciences, 2020(11).DOI:10.1073/PNAS.1920251117.
[2] Békés, M., Langley, D.R. & Crews, C.M. PROTAC targeted protein degraders: the past is prologue. Nat Rev Drug Discov 21, 181–200 (2022). https://doi.org/10.1038/s41573-021-00371-6
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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