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Part:BBa_K4600001:Design

Designed by: Kavirang Biswas   Group: iGEM23_Warwick   (2023-09-21)
Revision as of 16:14, 26 September 2023 by KavirangB (Talk | contribs) (→‎References)

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AcrB is a part of a multidrug efflux pump system found commonly in E.coli.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2830
    Illegal XhoI site found at 773
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
    Illegal NgoMIV site found at 859
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 1864
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was already codon optimised.


Source

The pump sequence used here, originates from the genomic sequence of E.coli K12, and was mapped in 1993.

References

Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol. 1993 Oct;175(19):6299-313. doi: 10.1128/jb.175.19.6299-6313.1993. PMID: 8407802; PMCID: PMC206727.