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Part:BBa_K4600001:Design

Designed by: Kavirang Biswas   Group: iGEM23_Warwick   (2023-09-21)
Revision as of 16:14, 26 September 2023 by KavirangB (Talk | contribs) (→‎Source)


AcrB is a part of a multidrug efflux pump system found commonly in E.coli.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2830
    Illegal XhoI site found at 773
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
    Illegal NgoMIV site found at 859
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 1864
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was already codon optimised.


Source

The pump sequence used here, originates from the genomic sequence of E.coli K12, and was mapped in 1993.

References