Part:BBa_K4905019
The Ompf sequence is a signaling peptide allowing for translocation of a protein to the periplasm
The E.coli K-12 strain possesses a protein, called the outer membrane protein F (Ompf). The precursor of this protein contains a signaling peptide 22 amino acids in length, which is the Ompf signaling sequence[1]. This sequence is used for translocating proteins from the cytoplasm to the periplasm[2]. For this reason, the Ompf sequence can be fused to other proteins for the secretion into the periplasm[3].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Inokuchi K, Mutoh N, Matsuyama S, Mizushima S (1982) Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res 10:6957-6968 [2] Vlasuk GP, Inouye S, Ito H, Itakura K, Inouye M (1983) Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli. J Biol Chem 258:7141-7148 [3] Yamamoto, T, Okawa N.,Endo T., and Kaji A. (1991) Expression of chimeric ras protein with OmpF signal peptide in Escherichia coli: localization of OmpF fusion protein in the inner membrane. Appl Microbiol BiotechnoZ 35:615-621
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