Composite
Part:BBa_K243012:Design
Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-14)
Strep-DigA-Middle Linker-Fok_i
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 278
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The choice of the linker, lipocalin tag and purification tag allows us serval different combinations.
Source
LOCUS Strep+Dig+middel 3625 bp DNA circular SYN 13-OCT-2009
DEFINITION Ligation of Strep+Dig+middellinker+FokI into pMA2_NgoMIV_SpeI
ACCESSION Strep+Dig+middel
KEYWORDS .
SOURCE Unknown.
ORGANISM Unknown
Unclassified.
REFERENCE 1 (bases 1 to 3625)
AUTHORS Self
JOURNAL Unpublished.
COMMENT SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI
Fragment ends: SpeI and NgoMIV
Fragment size: 2412
Insert molecule: Strep+Dig+middellinker+FokI
Fragment ends: NgoMIV and SpeI
Fragment size: 1213
FEATURES Location/Qualifiers
CDS 129..989
/gene="AmpR"
/SECDrawAs="Gene"
CDS 2008..3222
/gene="ORF-1"
/SECDrawAs="Gene"
CDS 2014..2041
/gene="'Strep-Tag'"
/SECDrawAs="Gene"
CDS 2044..2599
/gene="'Dig'"
/SECDrawAs="Gene"
CDS 2602..2628
/gene="'middelLinker38"
/SECDrawAs="Gene"
CDS 2632..3219
/gene="'FokI"
/SECDrawAs="Gene"
ORIGIN
1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa
2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac
2101 tggtcccagt accacggcaa gtggtggcag gtggccgctt atcccgacca catcaccaag
2161 tacggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtcccgg
2221 tacagcgtga tccacggcaa agagtacttc agcgagggca ccgcctaccc tgtgggcgac
2281 agcaagatcg gcaagatcta ccacagctac accatcggcg gcgtgaccca ggtgggcgtg
2341 agcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacttttg cagatacgac
2401 gaggacaaga agggccactg ggacgccgtg tgggtgctgt cccggtccat ggtgctgacc
2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag
2521 aaactggtgt acagcgactt ctccgaggcc gcctgcaaag tgaacaacag caactggtcc
2581 cacccccagt tcgaaaagac cggcggtggt tctggtggtg gttctggtac cggcaaatct
2641 gaactggagg agaaaaaatc cgagctgcgc cacaaactga aatatgtgcc tcacgagtat
2701 atcgaactga tcgagatcgc ccgtaatagt acccaagacc gtatcctgga aatgaaagtg
2761 atggagttct tcatgaaagt ctatggctat cgtggcaaac atctgggtgg tagccgtaaa
2821 ccagcaggtg ccatttatac cgttggttcc ccgatcgatt atggcgttat cgtggccaca
2881 aaagcgtatt ctggcggtta taatctgccg attggtcagg ctgatgagat ggaacgttat
2941 gtggaagaga atcagacccg taacaaacat ctgaacccga acgaatggtg gaaagtgtat
3001 ccgtcaagtg tcaccgagtt caaatttctg ttcgtgagcg gccactttaa aggcaactat
3061 aaagcccagc tgactcgtct gaaccatatc accaatagca atggggcagt gctgagtgtt
3121 gaggaactgc tgatcggtgg agaaatgatc aaagcaggca ccctgactct ggaagaagtt
3181 cgccgtaaat tcaacaatgg cgagatcaat tttaccggtt aatactagta gcggccgctg
3241 cagagctcat ggcgcgccta ggccttgacg gccttccgcc aattcgccct atagtgagtc
3301 gtattacgtc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
3361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
3421 aggcccgcac cgaaacgccc ttcccaacag ttgcgcagcc tgaatggcga atgggagcgc
3481 cctgtagcgg ccactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
3601 caaaatatta acgcttacaa tttag
//