Part:BBa_K4390110

L2NC tag expression (control)

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

A truncated version of the L2 ribosomal protein from E. coli, for fusion to N-terminal of a protein using JUMP assembly (N-terminal 1-60 and C-terminal 203-273 amino acids of L2 [silica-binding regions of L2 only] 130 amino acids). This tag contains just the N and C-terminal regions of L2 which were shown to have silica binding capacity, allowing the use of a smaller tag without compromising on binding affinity. From literature, the dissociation constant between L2NC silica tag and silica beads is 1.7nM (Kim et al., 2020). The N-terminal L2NC silica tag is inspired by 2021 Edinburgh OG teams (BBa_K3946002).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 108
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Kim S, Joo K, Jo B, Cha H. Stability-Controllable Self-Immobilization of Carbonic Anhydrase Fused with a Silica-Binding Tag onto Diatom Biosilica for Enzymatic CO2 Capture and Utilization. ACS Applied Materials & Interfaces. 2020;12(24):27055-27063.