Composite

Part:BBa_K4390109

Designed by: Zhongyi Liang   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-21)
Revision as of 14:04, 12 October 2022 by Zachary (Talk | contribs) (→‎Usage and Biology)


Car9 tag expression (control)

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

Car9 is a short silica-binding tag to add to the N-terminal of a protein using JUMP assembly, including a short alanine-rich linker (AAAL). The tag facilitates immobilisation to silica surfaces with a dissociation constant (1 µM), enabling enzyme immobilisation or purification using silica-based spin columns. The advantage of using Car9 silica tag is its small size (1.87 kDa) would introduce smaller effect to the functional enzyme activity in theory (Coyle and Baneyx, 2014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None