Coding
TEV

Part:BBa_K4225009

Designed by: YAU Janviere   Group: iGEM22_HKUST   (2022-10-09)
Revision as of 13:32, 12 October 2022 by JanviereYau (Talk | contribs)

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TEV protease


HKUST 2022 Team used this TEV protease in conjunction with LVA degradation tag.
TEV Protease has 2 key functions :
- Degradation rescue of RFP to amplify output
- Induced degradation of GFP to reduce colour mixing


Through proteolytically cleaving the TEV protease cut site (ENLYFQ), TEV protease can posttranslationally modify expressed proteins



TEV as a basic part has been used to create composite parts:
Ptac-TEV-myc-Pc-RFP (BBa_K4225011)
Ptac-TEV-myc-Pc-RFP-CS-LVA (BBa_K4225012)
Ptac-TEV-Pc-GFP-LVA-CS (BBa_K4225018)
Ptac-TEV-Pc-GFP (BBa_K4225020)
Ptac-TEV -Pc-GFP-LVA-CS-IS (BBa_K4225019)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316
    Illegal SapI.rc site found at 664


[edit]
Categories
Parameters
None