Part:BBa_K4275032

PfuFerritin-Nb3

PfuFerritin--Nb3 is combination of a ferritin protein and the nanobodies displayed on the surface. Ferritin is a spherical iron storage protein composed of a self-assembled 24- subunit protein shell and it is commonly used in blood tests for iron levels in humans. Due to its large numbers of salt bridges and hydrogen bonds, ferritin is extremely heat-resistant and exhibits excellent biocompatibility in vivo. Due to its ability to self-assemble and because of its unique architecture, ferritin has been used to display peptides as well as larger proteins[1].

Usage and Biology

PfuFerritin-Nb3 can be used to increase binding affinity, thermal resistance and extending blood half-life in vivo of nanobodies. And because PfuFerritin has high heat stability it tends to be suitable for scale up experiments and translational studies[1]. Moreover, its long half-life also means that there are less modifications required to the protein which may affect the functional properties of the protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 547
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Fan, Kelong et al. “Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.” Analytical chemistry vol. 90,9 (2018): 5671-5677. doi:10.1021/acs.analchem.7b05217