Part:BBa_K4204014:Design

Quality control system + LysPBC5

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 907
  Illegal PstI site found at 31
  Illegal PstI site found at 367
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 907
  Illegal NheI site found at 1017
  Illegal NheI site found at 1099
  Illegal PstI site found at 31
  Illegal PstI site found at 367
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 907
  Illegal BglII site found at 916
  Illegal BamHI site found at 1794
  Illegal XhoI site found at 2122
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 907
  Illegal PstI site found at 31
  Illegal PstI site found at 367
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 907
  Illegal PstI site found at 31
  Illegal PstI site found at 367
  Illegal NgoMIV site found at 346
  Illegal NgoMIV site found at 1846
  Illegal AgeI site found at 496
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.

Source

Eukaryotic translation system.

References

[1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.

[2] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001