Part:BBa_K4275031
PfuFerritin-wt
Ferritins are present in a wide variety of organisms: bacteria, archaea, fungi, plants, insects and vertebrates. Their main role is presumably iron storage; excess of Fe2+ from a cell environment is internalized and then taken up by ferritin, oxidized, and stored as ferrihydride in its cavity until the cell needs it.
Ferritin has properties of an longer half life and good thermostability meaning that it can be used for nanobody displays. Most importantly in our project, ferritin can be used to magnetophoresis which is plays the role of recollecting microbials in a reactive solution. Because ferritin mainly exists in the cytoplasm of microbials, it the iron component in the cell functions to locate the bacteria in our project.
Usage and Biology
The main role of ferritin is iron storage. excess of Fe2+ from a cell environment is internalized and then taken up by ferritin, oxidized, and stored as ferrihydride in its cavity until the cell needs it. Upon addition of reducing equivalents, iron is released. This function of ferritins is a key part of the homeostasis machinery of higher organisms that ensures the right balance of iron in the organism. Regulating iron levels is important given that iron needed but excess levels of iron leads to oxidative stress in higher organisms.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. "Fan, Kelong et al. “Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.” Analytical chemistry vol. 90,9 (2018): 5671-5677. doi:10.1021/acs.analchem.7b05217
2. Li, Thomas L et al. “Engineering a Genetically Encoded Magnetic Protein Crystal.” Nano letters vol. 19,10 (2019): 6955-6963. doi:10.1021/acs.nanolett.9b02266
3. Tatur, Jana et al. “A highly thermostable ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus.” Extremophiles : life under extreme conditions vol. 10,2 (2006): 139-48. doi:10.1007/s00792-005-0484-x
4. Aubry, Mary et al. “Engineering E. coli for Magnetic Control and the Spatial Localization of Functions.” ACS synthetic biology vol. 9,11 (2020): 3030-3041. doi:10.1021/acssynbio.0c00286"
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