Device

Part:BBa_K174008:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-10)
Revision as of 20:25, 10 October 2009 by Registry (Talk | contribs)

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SmtA metallothionein protein with CotC and Gfp fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1046


Design Notes

It is integrated to B. subtilis using the homology between cotC fusion and the native copy of cotC on the chromosome.


Source

smtA sequence is taken from E. coli

References