File:Level 1 Results TU1 (K4388005).png
Revision as of 13:07, 11 October 2022 by Tomaslopes (Talk | contribs) (Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion react...)
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Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 20:02, 11 October 2022 | | 1,626 × 902 (885 KB) | Tomaslopes (Talk | contribs) | BBa_K4388000 replaced by BBa_K4388013 (as the latter correctly contains the lacO site between the promoter and RBS for expression control with IPTG). |
| 13:07, 11 October 2022 |  | 1,700 × 1,000 (1 MB) | Tomaslopes (Talk | contribs) | Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion react... |
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