Regulatory

Part:BBa_K4317002

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-11)
Revision as of 11:18, 11 October 2022 by Envien mc (Talk | contribs)


Pmglv

Maltose-inducible promoter usable in Bacillus subtilis

800px-Pmglv.png

Usage and Biology

800px-Promoter_strength.png

Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)

References

Ming, Yang M., et al. "Development of a Bacillus subtilis expression system using the improved Pglv promoter." Microbial cell factories 9.1 (2010): 1-8. Song, Yafeng, et al. "High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization." Journal of agricultural and food chemistry 65.12 (2017): 2540-2548.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None