Composite

Part:BBa_K4452012

Designed by: iGEM22_Hopkins   Group: iGEM22_Hopkins   (2022-09-30)
Revision as of 04:40, 10 October 2022 by KalenClifton (Talk | contribs) (add GFP)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Ferritin with prcpHsc70-1 transit peptide

Pyrococcus furiosus ferritin (PFt), an efficient iron storage protein, linked to GFP with expression under a columella cell-specific promoter, PLT-2, and with an N-terminus signal peptide to imports the ferritin into statoliths.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2370
    Illegal BsaI.rc site found at 2649


[edit]
Categories
Parameters
None