Plasmid_Backbone

Part:BBa_K4452010:Design

Designed by: Juan Antonio Garcia, Fabio Pasin   Group: iGEM22_Hopkins   (2022-09-30)
Revision as of 16:24, 9 October 2022 by KalenClifton (Talk | contribs) (replace missing design page)

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pLX-B3Ω1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 616
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
    Illegal NgoMIV site found at 954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 596


Design Notes

none


Source

https://www.addgene.org/160643/

References

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins. Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA. ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 10.1021/acssynbio.6b00354. Epub 2017 Jun 28. 10.1021/acssynbio.6b00354 PubMed 28657330