Part:BBa_K4286101

Oscillator device for improved version of timed suicide switch

2022 SZU-China has designed the second generation of timed suicide switch. The following improvements have been made to the second generation oscillator: the MazF expression device has been added; the LVA degradation tag has been removed. These changes create a concise and elegant oscillator with highly regular and longer period oscillations.

Usage and Biology

Gene oscillation is a gene regulation mechanism, and the amplitude and period of oscillation reflect the gene expression. Its principle is that three gene modules whose encoded repressors inhibit each other are connected in series to form negative feedback, and the periodic change of the content of repressor is realized by the inhibition and deinhibition of gene modules. The oscillator contains three repressor proteins: TetR from the Tn10 transposon, λ cI from bacteriophage λ, and LacI from the lactose operon. Each repressor carries an LVA degradation tag at the C-terminus. What's more, the oscillator2.0 has a longer oscillation period and additionally has the function of expressing Toxin MazF. The oscillator2.0 is encoded on a low copy plasmid pSB3C5.

Sequencing

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2662
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]