Part:BBa_K4325015

J23102-RBS003422-gshF-T0

J23102-B0034-gshF-T0

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 223
  Illegal EcoRI site found at 805
  Illegal EcoRI site found at 1240
  Illegal EcoRI site found at 1738
  Illegal PstI site found at 64
  Illegal PstI site found at 94
  Illegal PstI site found at 451
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 223
  Illegal EcoRI site found at 805
  Illegal EcoRI site found at 1240
  Illegal EcoRI site found at 1738
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 64
  Illegal PstI site found at 94
  Illegal PstI site found at 451
  Illegal NotI site found at 1883
  Illegal NotI site found at 2083
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 223
  Illegal EcoRI site found at 805
  Illegal EcoRI site found at 1240
  Illegal EcoRI site found at 1738
  Illegal XhoI site found at 2064
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 223
  Illegal EcoRI site found at 805
  Illegal EcoRI site found at 1240
  Illegal EcoRI site found at 1738
  Illegal PstI site found at 64
  Illegal PstI site found at 94
  Illegal PstI site found at 451
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 223
  Illegal EcoRI site found at 805
  Illegal EcoRI site found at 1240
  Illegal EcoRI site found at 1738
  Illegal PstI site found at 64
  Illegal PstI site found at 94
  Illegal PstI site found at 451
  Illegal NgoMIV site found at 1665
  Illegal NgoMIV site found at 2296
  
• 1000
  COMPATIBLE WITH RFC[1000]

2022 SZPT-China

Biology

This composite part is a generator consisting of a J23102 (BBa_J23102) promoter and gshF (BBa_K4325003).

Usage

The J23102 promoter and gshF were connected and inserted into the pSEVA331 expression vector so that gshF expressed the novel bifunctional enzyme GshF, which directly catalyze the synthesis of glutathione by the three amino acids of Cys, Glu and Gly.