Coding
CycA

Part:BBa_K4339000:Design

Designed by: Grace Poulton   Group: iGEM22_Exeter   (2022-07-06)
Revision as of 14:59, 8 October 2022 by ChloeS (Talk | contribs) (→‎Design Notes)


Non-polar amino acid transporter (CycA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was modified to remove forbidden restriction sites to allow for Type IIS cloning.

Source

The CycA sequence originates in a K-12 strain of E. coli.

References