Composite

Part:BBa_K4164017

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)
Revision as of 12:41, 8 October 2022 by JieYang (Talk | contribs)


Inductive expression of FXR-ddRFPA1-RXR-ddRFPB1

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7lac promoter from pET-29a+(Novagen).

The composite part can be directly imported into plasmid and express FXR-ddRFPA1 and RXR-ddRFPB1 induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1964
    Illegal NheI site found at 2232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3846
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
    Illegal AgeI site found at 1481
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 836
    Illegal SapI.rc site found at 3435


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Categories
Parameters
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