Part:BBa_K4413993

PGroES-RiboJ-DrRBS-gabY

gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate.

Introduction

BBa_K3560005 contains RBS and gabY genes. We plan to improve this part, and constructed the improved part (BBa_K4413993). We inserted a RiboJ sequence in the upstream of gabY and downstream of the PGroES promoter. In addition, we described the functions of this part in Deinococcus radiodurans (DR), including the growth curve and the ability to decrease pH and dissolve phosphate.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 476
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 190
  Illegal NgoMIV site found at 390
  Illegal NgoMIV site found at 482
  Illegal NgoMIV site found at 648
  
• 1000
  COMPATIBLE WITH RFC[1000]