Composite

Part:BBa_K4255008

Designed by: Zifu Tang   Group: iGEM22_SHSBNU_China   (2022-10-08)
Revision as of 10:01, 8 October 2022 by Registry (Talk | contribs)

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This is a gene to code 4CL-CHS, CHI protein

We constructed the first three enzymes on the pETDUET vector, in which 4CL was linked to CHS via linker to form a fusion protein and CHI was expressed independently. The function of the enzymes can be listed below: 4CL: catalyze phenylalanine into 4-coumaryl coenzyme A CHS: catalyze 4-coumaryl coenzyme A into chalcone CHI: catalyze chalcone into flavanone


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None