Part:BBa_K4199002
HrtR
The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]
Usage and Biology
The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 249
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 249
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 249
- 1000COMPATIBLE WITH RFC[1000]
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