Composite
Part:BBa_K4385014
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Lead constitutive surface display
Lead constitutive surface display consists of the constitutive promoter J23100, the lead-inducible regulatory protein PbrR and the anchoring protein Lpp-OmpA. J23100 continuously expresses PbrR for lead ion adsorption, and Lpp-OmpA is used as an anchoring protein to anchor PbrR on the surface of E. coli to construct a whole-cell biosorption device for lead.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1120
Illegal NheI site found at 1143
Illegal SpeI site found at 938
Illegal PstI site found at 880 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1255
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 671
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Categories
Parameters
None |