Composite

Part:BBa_K4385014

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-01)
Revision as of 15:41, 6 October 2022 by Minan (Talk | contribs)


Lead constitutive surface display

Lead constitutive surface display consists of the constitutive promoter J23100, the lead-inducible regulatory protein PbrR and the anchoring protein Lpp-OmpA. J23100 continuously expresses PbrR for lead ion adsorption, and Lpp-OmpA is used as an anchoring protein to anchor PbrR on the surface of E. coli to construct a whole-cell biosorption device for lead.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1120
    Illegal NheI site found at 1143
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 671


[edit]
Categories
Parameters
None