Composite
PzraP-GFP
Part:BBa_K4385013
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-03)
Zinc sensor
The zinc sensor part consists of the PzraP promoter, ZraSR two-component membrane associated sensor kinase system and GFP. ZraSR consists of histidine kinase ZraS, cytoplasmic transcription factor ZraR and periplasmic accessory protein ZraP, responsible for sensing the presence of external Zn2+. The ZraSR system regulates the expression of zraP, which encodes a periplasmic zinc-binding protein, in response to high concentrations of extracellular zinc ions; phosphorylated ZraR triggers divergent transcription of zraP and zraSR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 907
[edit]
Categories
Parameters
None |