Composite
PcusC-GFP
Part:BBa_K4385011
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Lead sensor
A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 was incorporated into E. coli in conjunction with an engineered downstream GFP (green fluorescence protein), which allowed for highly sensitive and selective whole-cell detection of lead ions.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 462
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 462
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 462
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 462
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI.rc site found at 1394
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Categories
Parameters
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