Plasmid

Part:BBa_K4204007

Designed by: Chujing Wu   Group: iGEM22_BNDS_China   (2022-10-05)
Revision as of 17:38, 5 October 2022 by WuChujing (Talk | contribs) (→‎Quality control system + LysPBC5)

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Quality control system + LysPBC5 Ori

Description

The quality control system is added to LysPBC5. Protein quality control systems include a switch at the 5’ end of an mRNA and a corresponding trigger sequence with reverse complementary to the switch at the 3’ end of the mRNA. When the Cis-trigger appears, the switch will bind with the trigger preferentially because the Cis-trigger has a higher affinity with the switch. Or, the switch binds with its complementary sequence and clocks the RBS inside a neck-ring structure; this blocks the transcription initiation. The QC system applied to the LysPBC5 increase the amount of functional protein. Other IGEM teams can use the tested ProQC system for full-length protein expression to increase the amount of functional protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1004
[edit]
Categories
Parameters
None