Project

Part:BBa_J37032:Experience

Designed by: John Sy   Group: iGEM06_Imperial   (2006-08-17)
Revision as of 11:03, 10 September 2009 by Jenni (Talk | contribs) (→‎User Reviews)

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Applications of BBa_J37032

User Reviews

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Aberdeen_Scotland 2009

This part was used to test our LuxR/LuxI operon (K182200). For that purpose J37032 has been transformed in SCS1 E.coli cells as negative control and furthermore SCS1 cells have been transformed with J37032 and K182200 (called pIR in below figure). A second control was the transformance of SCS1 cells with K182200 only. It was found that this testing construct produces the same amount of GFP with or without the presence of LuxR. J37032 is hence not responsive to LuxR or the pLux promoter is extremely leaky and thereby should not be used to test quorum sensing. The Figure below shows the negative controls (J37032 alone and pIR alone) as well as both biobricks together under a light and fluorescence microscope.

J37TEST figure.jpg



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