Regulatory

Part:BBa_K4140017

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 11:11, 4 October 2022 by Ahmed Wael (Talk | contribs) (→‎Usage)


CMV promoter

Part Description

CMV (cytomegalovirus) promoter is a strong constitutive promoter commonly used in mammalian expression systems to produce high levels of expression, However CMV promoter is on of the most variable types of promoter as it is highly variable between different cell types such as being strong in some cell types and being much weaker in other types

Usage

We used a mammalian expression systems to regulate the expression of Cas12g which have a remarkable role on tuning and controlling our circuit activity and the levels of PAH to be constant within normal level to avoid overexpression of cas12g

Literature Characterization

Figure.1 Effect of different promoter on transfection efficiency and transient transgene expression using eGFP antibody analysis.




















References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None