Coding
WaaL Ligas

Part:BBa_K200003

Designed by: Charles Fracchia, James Field   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 16:07, 28 August 2009 by CharlesFracchia (Talk | contribs)

Rfal

Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the lipid A core, in K-12 it links colanic acid to the lipid A core. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 754
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None