Reporter

Part:BBa_K3977002

Designed by: Yilong Xu   Group: iGEM21_SCU-China   (2021-10-01)
Revision as of 06:50, 26 September 2022 by Tigress K (Talk | contribs)


mSarlet-I

mSarlet-I

Usage and Biology

Characterized by CAFA_China 2022

  • We conducted a simple test to see if our design met the expection.
  • We designed the genetic circuit that contains sulAp (sulA promoter), Amp30E, relE gene (RelE toxin), mScarlet-I (mSarlet-I) and 3WJ-Bro, and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device).
  • The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None