Part:BBa_K3861013

AIDA signal sequence

Signal sequence of the AIDA-I autotransporter derived from pRAIDA2.¹ Will be cleaved prior to secretion of the autotransporter. Must be used together with the autotransporter to allow display of a protein of interest (POI) on the bacterial surface. The gene encoding for the POI is to be inserted downstream of the signal sequence and upstream of the autotransporter. The AIDA-I autotransporter part can be found here: BBa_K3861016. We combined the parts to create a construct with the potential ability to display the recombinant lectin rBC2LCN (BBa_K3861001) on the surface of bacterial minicells in order to enable active targeting of MCF7 breast cancer cells by attaching to glycans on the cancer cell surface (BBa_K3861018).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 32
  

References

1. Yu, H. et al. Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells, ACS Synthetic Biology, (2021).