RNA
gard-337

Part:BBa_K3714000

Designed by: Yuxin Lu   Group: iGEM21_SJTU-BioX-Shanghai   (2021-08-30)
Revision as of 01:17, 22 October 2021 by Grus (Talk | contribs)


Gardiquimod biosensor 337

The template of a new self-cleaving aptazyme[1], which keeps itself intact with gardiquimod present, and catalyzes the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod. The feature "Aptazyme" is necessary, and two A-rich oligonucleotides are added at both terminal to avoid unwanted secondary structure.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714000.png
Figure.1 | Verification of biosensor gard-337. An in vitro transcription system was employed to test if this biosensor can response gardiquimod signal. The product was run on a denatured PAGE.

Reference
[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. & Smolke, C. D. A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nature Communications 12, 1437, doi:10.1038/s41467-021-21716-0 (2021).

[edit]
Categories
//rna/aptazyme
Parameters
efficiency10 μM gardiquimod inhibits 94.4% cleavage[1]