Part:BBa_J119449
N Clone of Mammal Ribozyme
The N Clone of Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a five nucleotide (nt) base change from the Mammal Ribozyme (J119446). At nt 77-81, the original ribozyme sequence of CATAA was edited to AATAA. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for the original Mammal Ribozyme is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Charcterization and improvement in 2021 by SJTU-BioX-Shanghai
In 2021, SJTU-BioX-Shanghai improved the sensibility of this part, see BBa_K3714004 for more details. In short, we improved the performance (cleave fraction) with theophylline present in vitro by introducing some mutation in the loops of aptazyme.
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