Coding

Part:BBa_K3895016

Designed by: Ziying JIN   Group: iGEM21_SZ_SHD   (2021-10-21)
Revision as of 08:29, 21 October 2021 by Auskuld (Talk | contribs) (→‎Experiment details)


Optimized keratinase KerA

Introduction

This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3). The KERA (BBa_K1717171) is a basic part comprised from a synthesized KERA protein coding sequence. The gene can be expressed in cells by looking for clear areas of cell growth on AGAR plates of skimmed milk, and by observing keratin-containing substrates (feathers, hair, chemosynthetic keratin, etc.) growing with cells.

Plasmid Construction

T--SZ SHD--kera.png

Experiment details

• Throughput: 2500
• Organism: E. coli
• Strain type: BL21(DE3)
• Temperature: 37°C
• Media: 250 ml LB
• Period of time: 16 hours
• Plasmid backbone: pSB1C3

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 326
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1369
    Illegal SapI site found at 957
    Illegal SapI site found at 1260


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Categories
Parameters
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