Composite

Part:BBa_K3722009

Designed by: Gao Zhan   Group: iGEM21_NWU-CHINA-A   (2021-09-30)
Revision as of 08:08, 21 October 2021 by SaraK (Talk | contribs)


T7_Promoter-RBS-SttH-T7_Terminator

Flavin-dependent Trp halogenases use halide ions (X−) and FADH2 for halogenation，if you use it in cell-free systems, requiring supplementation of the high-cost cofactor FADH2 and additional cofactor regenerating enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1635
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 526
    Illegal AgeI site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None